The Extraction Of Dna From A Kiwi Fruit Run Through...

Title: The Extraction of DNA from a Kiwi Fruit Run Through Agarose Gel Electrophoresis. Introduction: In this experiment the use of gel electrophoresis and DNA extraction will be used to see that kiwi fruit has evidence of DNA. The aim is to be able to extract the DNA from a Kiwi Fruit then be able to load then visualize the DNA that was extracted through the use of Gel Electrophoresis. Materials: The materials used were Kiwi Fruit (peeled and cut into triangles), Plastic zip lock bag, I – 10 mL black and red auto pipette and tips, 1000mL of DNA extraction Buffer (combination of 95% water, 5% detergent and 2 table spoons of sugar), piece of Chux cloth, funnel, conical flask, 20 – 200 ÃŽ ¼L yellow and white auto pipette and tips, 15mL tube with lid, texta (permanent marker), Cold Ethanol, ice, container (for the ice), hook, Eppendorf Tube (1.5 mL in volume) containing 1 mL of 70% ethanol. Microcentrifuge, paper towel, distilled water, heat block (55Â °C), Eppendorf Tube (1.5 mL in volume) containing dye, Agarose Gel and a power station that the Gel goes in. Method: First the Kiwi Fruit was placed in the zip lock bag and sealed. The sample was then squished using for 3 minutes by hand, using the 1 – 10 mL auto pipette and tip 10mL of DNA extraction buffer was drawn and placed into the zip lock bag with the squished Kiwi Fruit, discard the tip, and then squished of an extra 2 minutes (trying not to create too many bubbles). Once the solution is combined the Chux cloth